Abstract
AbstractTwo similar methods, normal phase high performance liquid chromatography and silica gel thin‐layer chromatography with densitometry, were applied for the separation and quantitative determination of the diterpene glycosides stevioside and rebaudioside A in the leaves of the plant Stevia rebaudiana Bertoni. Results were compared and found to be very close in all basic features: resolution, accuracy and reproducibility. Thin‐layer chromatrography was found to be more suitable in case of phytochemical screening of large number of samples.
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