Abstract
Study aimed to isolate and purify compounds from the stems and leaves of Panax quinquefolius. By employing a highly innovative separation technique that combined multistage countercurrent chromatography (MRCC), high-speed countercurrent chromatography (HSCCC), and an advanced online-storage inner-recycling countercurrent chromatography (OS-IRCCC) mode for the first time, 12 compounds were successfully isolated, including 10 ginsenosides and 2 flavonoids. First, the crude extract was fractionated into five parts using D101 MRCC, with HPLC analysis revealing that 40% and 60% ethanol eluate contained the highest compound diversity. Overall, 40% ethanol eluate was separated using the solvent system of EtOAc/n-BuOH/H2O (2:1:3, v/v), whereas 60% ethanol eluate underwent traditional countercurrent chromatography coupled with OS-IRCCC separation using the solvent system of methyl tert-butyl ether (MTBE)/n-BuOH/ACN/H2O (4:2:3:8, v/v). Ultimately, various compounds were obtained, including kaempferol 3-O-β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-7-O-α-l-rhamnopyranoside (13.2mg), ginsenoside Rc (7.4mg), 20(R)-ginsenoside Rh1 (7.2mg), ginsenoside Re (12.3mg), kaempferol 3-O-β-d-glucopyranosyl-(1→2)-β-d-galactopyranoside (14.1mg), ginsenoside Rb1 (8.2mg), ginsenoside Rb2 (17.5mg), ginsenoside Rb3 (27.3mg), ginsenoside Rg1 (13.3mg), ginsenoside Rg2 (9.7mg), ginsenoside Rd (11.4mg), and pseudo-ginsenoside F11 (16.7mg). This research highlights the efficacy of the novel separation technique in isolating and purifying valuable compounds from P. quinquefolius stems and leaves.
Published Version
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