Abstract
The polysaccharide fractions amylose (AM) and amylopectin (AP) were obtained from both a native and an acid‐thinned potato starch sample. The starches were dissolved using an autoclaving and an alkaline dissolution process and fractionated by complexing the AM with 1‐butanol. The fractions were comprehensively characterized by means of size exclusion chromatography‐multi angle laser light scattering (SEC‐MALS) and iodine binding experiments. The fractions’ high purity was generally proved. Molecular degradation of both starch fractions due to autoclaving was evident and more pronounced for the acid modified sample by trend. The SEC‐chromatograms confirmed the elution of the linear AM‐fractions entirely accompanied by branched AP. Complete enzymatic debranching the starch sample prior to chromatography and mathematical peak separation was an alternative method for the molecular characterization of the AM‐fraction. However, this procedure is restricted to the non‐branched AM and found particularly suitable for partial molecular degraded starch (acid‐thinned).
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