Separation and Identification of Oligomeric-Hindered Amine Light Stabilizer Uvasorb HA 88 by High-performance Liquid Chromatography (HPLC) Coupled With Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALSI-TOF-MS)

Abstract

Uvasorb HA 88 is widely used as a light stabilizer to prevent plastic polymer degradation. Its high molecular weight and oligomeric characters provide challenge for quality control. In this study, high-performance liquid chromatography (HPLC) coupled with electrospray ionization time of flight mass spectrometry (ESI-TOF MS) was applied for separation and detection of Uvasorb HA 88. The synthesis scheme was deduced and confirmed by the characterization of Uvasorb HA 88 products from different batches. Direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was employed for the detailed characterization of chemical composition of Uvasorb HA 88. Furthermore, a reliable reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with MALDI-TOF MS method was established and the weight-average and number-average molecular masses were calculated. The results revealed that six oligomers with repeat unit numbers from 1 to 4 were present in Uvasorb HA 88. A molecular weight of up to 6808 g mol−1 was detected and two new series of oligomers were reported for the first time. Different series of oligomers and positional isomers were observed in Uvasorb HA 88. This work provides a suitable method to evaluate the technical grade of Uvasorb HA 88 as well as comprehensive characterization of complex oligomeric hindered amine light stabilizers.