Abstract
Nishiki, T., Sato, I. and Kataoka, T., 1990. Separation and concentration of proteins with liquid-liquid extraction using reversed micelles. Protein extractions using Aerosol OT (AOT)-isooctane reversed micelle solutions have been studied to explore the potential for separating and enriching proteins with the reversed micellar extraction. The effects of pH, ionic strength and chlorides in a bulk aqueous phase, and AOT concentration in an organic phase on the partitioning of lysozyme and myoglobin and the solubilization of water were examined in detail. Lysozyme could be enriched into the reversed micellar phase up to thirty times the aqueous feed concentration. Lysozyme in the reversed micellar phase was efficiently stripped with potassium chloride solutions, whose concentration was 1-2 kmol/m 3 and pH was 11-12. The results indicated that the separation of two proteins and the concentration of lysozyme are capable.
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