Abstract

A process is described which separates two hemagglutinins from vaccinia-infected chorioallantoic membrane suspensions by centrifugation in a suspending medium of density 1.063. The properties of these hemagglutinins have been studied and the results indicate that one hemagglutinin is virus specific and the other hemagglutinin is a normal tissue agglutinin also present in uninfected membrane suspensions. Criteria are presented for the differentiation of the two hemagglutinins and for the determination of the virus specificity of one of the hemagglutinins.

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