Separation and biochemical characterization of blood group N antigen precursor glycoproteins with thomsen-friedenreich (T) activity, T-active glycoproteins and N antigen precursor glycoproteins from ascites of primary ovarian cancer patients

Abstract

1. 1. Three perchloric acid-soluble fractions from ascites of three primary ovarian cancer patients were subjected to Sephacryl S-300 gel filtration, respectively, and three Fr. 1 which were eluted in the vicinity of void volume as minor fractions, were then separated by a systematic affinity chromatography using Vicia unijuga lectin-Sepharose CL-4B column and Arachis hypogaea lectin-Sepharose CL-4B column into three glycoproteins, blood group N antigen precursor glycoprotein with Thomsen-Friedenreich (T) activity, T-active glycoprotein and N antigen precursor glycoprotein, respectively. 2. 2. These nine glycoproteins separated in yields of 0.1–1.3 mg per 100 ml of ascites, were demonstrated to be mucin-type glycoproteins with M w of 1,791,000–4,921,000 and contained 33.8–56.1% carbohydrates.