Abstract
Herein we report a sensor array based on quartz crystal microbalance (QCM) to simultaneously detect two biomarkers, namely low-density lipoprotein (LDL), and high-density lipoprotein (HDL). Selective recognition takes place through molecularly imprinted polymers (MIP) with both MIPs and corresponding non-imprinted polymer (NIP) as reference electrode. Sensor array performs highly appreciably in term of selectivity to LDL and HDL (defined through its cholesterol LDL-C, and HDL-C) when operated in 10 mM PBS. The sensor response time is less than 15 min. Furthermore, coefficients of variation indicating precision of our sensor array are at 2%–8%.
Highlights
Molecular imprinting constitutes a procedure synthesizing polymers containing specific recognition sites that are complementary to the template concerning shape and positioning of functional groups, namely molecularly imprinted polymers (MIPs)
Serum concentrations of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) particles in clinical assessment are usually determined in term of the amount of cholesterol within lipoprotein particles, namely LDL-C and HDL-C, respectively
Tri gold-electrode patterns of 10 MHz quartz crystal microbalance (QCM) were spin-coated with oligomer mixtures containing methacrylic acid (MAA) and N-vinylpyrrolidone (VP) cross-linked by ethylene glycol dimethacrylate (EGDMA), followed by imprinting with LDL and HDL which led to LDL-MIP and HDL-MIP, respectively
Summary
Molecular imprinting constitutes a procedure synthesizing polymers containing specific recognition sites that are complementary to the template concerning shape and positioning of functional groups, namely molecularly imprinted polymers (MIPs). They are established as synthetic receptors with binding ability comparable to natural receptors, but provide longer stability in harsher conditions including high temperature, non-physiological pH, and organic solvents, low manufacturing cost, and straightforward synthesis [1]. Serum concentrations of LDL and HDL particles in clinical assessment are usually determined in term of the amount of cholesterol within lipoprotein particles, namely LDL-C and HDL-C, respectively. Cholesterol contents in each lipoprotein class are analyzed using a homogenous enzymatic colorimetric assay. We report preliminary results for the design of LDL and HDL sensor array which can assess both lipoproteins simultaneously
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call