Abstract
RodZ is a cytoskeletal protein associated with bacterial cell shape. It is a transmembrane protein located on the plasma membrane, and it binds to another cytoskeletal protein MreB. Deinococcus grandis contains a rodZ homolog. Although D. grandis is rod-shaped, it becomes spherical in shape when the rodZ homolog is disrupted. The rodZ deletion mutant was treated with lysozyme to generate spheroplasts. The spheroplasts enlarged in medium containing calcium chloride and penicillin. The rodZ deletion mutant spheroplasts were more sensitive to calcium ions than wild type. Cell and cytoplasm sizes of enlarged spheroplasts of the rodZ deletion mutant tended to be larger than those of wild type. Thus, disruption of rodZ enhances plasma and outer membrane expansion in D. grandis spheroplasts.
Highlights
The genus Deinococcus consists of both bacilli and cocci species [1]
RodZ is a cytoskeletal protein associated with bacterial cell shape
Lipidomics of total cells showed that D. grandis has a unique lipid composition, as D. grandis lacks phosphatidylethanolamine (PE) and phosphatidylglycerol (PG), which are commonly found in other bacteria [7]
Summary
The genus Deinococcus consists of both bacilli and cocci species [1]. Deinococcus lacks lipopolysaccharides on its outer membrane [2,3]. Lipidomics of total cells showed that D. grandis has a unique lipid composition, as D. grandis lacks phosphatidylethanolamine (PE) and phosphatidylglycerol (PG), which are commonly found in other bacteria [7] This is consistent with the fact that Deinococcus radiodurans has glycolipids and glycophospholipids but lacks PE and PG due to the lack of biosynthetic genes [10,11,12]. Lipid composition changes during enlargement of D. grandis spheroplasts [7] This suggests that regulation of biosynthesis of the outer and plasma membranes may change during enlargement. In order to elucidate whether the MreB-RodZ cytoskeleton system inhibits plasma membrane expansion, we disrupted the rodZ homolog in D. grandis. The rodZ deletion mutant (∆rodZ) was treated with lysozyme to induce spheroplast enlargement. We compared enlargement levels and morphology of ∆rodZ with those of the wild type
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