Sensitivity of Botrytis cinerea Isolates Complex to Plant Extracts.

Lina Dėnė,Alma Valiuškaitė

doi:10.3390/molecules26154595

Lina Dėnė, Alma Valiuškaitė

Open Access

https://doi.org/10.3390/molecules26154595

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Abstract

New agricultural strategies aim to reduce the use of pesticides due to their damage to the environment and humans, and the caused resistance to pathogens. Therefore, alternative sources of antifungal compounds from plants are under investigation lately. Extracts from plants have a wide composition of chemical compounds which may complicate the development of pathogen resistance. Botrytis cinerea, causing grey mould, is an important horticultural and ornamental pathogen, responsible for the relevant yield and quality losses. B. cinerea isolated from a different plant host may differ in the sensitivity to antifungal substances from plants. Assessing the importance of research covering a wide range of pathogens for the rapid development of biopesticides, this study aims to determine the sensitivity of the B. cinerea isolate complex (10 strains) to plant extracts, describe morphological changes caused by the extract treatment, and detect differences between the sensitivity of different plant host isolates. The results showed the highest sensitivity of the B. cinerea isolates complex to cinnamon extract, and the lowest to laurel extract. In contrast, laurel extract caused the most changes of morphological attributes in the isolates. Five B. cinerea isolates from plant hosts of raspberry, cabbage, apple, bell pepper, and rose were grouped statistically according to their sensitivity to laurel extract. Meanwhile, the bell pepper isolate separated from the isolate complex based on its sensitivity to clove extract, and the strawberry and apple isolates based on their sensitivity to cinnamon extract.

Highlights

New strategies for reducing pesticide use are an inevitable, scientifically and practically demanding challenge
This study investigated the sensitivity of the B. cinerea isolates complex to plant extracts under different concentrations
The isolates complex showed the highest sensitivity to cinnamon extract

Summary

Introduction

New strategies for reducing pesticide use are an inevitable, scientifically and practically demanding challenge. In the case of fungicides, it is already known that they have adverse effects on the environment and humans [1,2]. They caused pathogen resistance [3,4]. Widespread disease control methods can become ineffective in the long run. With all the knowledge about pesticide residues in food, consumers tend to choose higher quality and more environmentally and human-safe products [5]. Alternative sources of antifungal substances are under investigation lately [6,7,8,9,10,11]. One alternative source is active compounds obtained from plants [12], such as botanical pesticides

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