Abstract
The sensitivity enhancement of wavelength modulation surface plasmon resonance (SPR) biosensor was described by improving the baseline solution that made the resonant wavelength move to longer wavelength. The baseline solutions here used are ethanol solutions of 1.0%, 2.0%, 4.0% and 6.0%. The higher is the concentration of the ethanol solution, the longer is the resonant wavelength, and the lower is the concentration of the analyte that could be detected. The heat shock protein (Hsp) 70 are studied and when the baseline solutions used are 1.0%, 2.0%, 4.0% and 6.0% ethanol solutions, the lowest concentrations of Hsp 70 that can be detected are 1.00, 0.50, 0.20 and 0.10 μg/ml, respectively.
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