Abstract
Temperature affects all enzymes simultaneously in a metabolic system. The enzyme concentration in a biochemical system can be considered as invariant under fast and small temperature change. Therefore, the total sensitivity of a steady state flux through a pathway with respect to the temperature can be expressed as: the apparent activation energy of a steady state pathway flux equals the sum of weighted activation energies of the individual reactions contributing to the flux, where the weighting factors are the flux control coefficients of these reactions in the context of the network. Correspondingly, since the elasticity of any enzyme with respect to temperature is always nonzero, only the reactions with a nonzero flux control coefficient contribute accordingly to the temperature sensitivity of the pathway.
Highlights
Temperature represents a major environmental factor that influences any living organism (Stephanopoulos et al, 1998; Nielsen et al, 2003; Roels, 1983; Zakhartsev et al, 2015; Esener et al, 1981)
The effect of temperature variations can be partially compensated through the kinetic regulatory mechanisms, which, are temperature dependent and the steady state flux through a pathway responds to the rapid temperature variations
Under fast and small temperature variations, the enzyme concentration is invariant, because under this assumption the change in enzyme concentration via gene expression is slower than the temperature change
Summary
Temperature represents a major environmental factor that influences any living organism (Stephanopoulos et al, 1998; Nielsen et al, 2003; Roels, 1983; Zakhartsev et al, 2015; Esener et al, 1981). In the same time a fast, random, small and short-term deviation of current temperature from its average value (i.e. fluctuations) always occur. The regulation of enzyme amounts through gene expression allows compensating the long-term effect of temperature variation thereby achieving homeostasis of a metabolic function (Ruoff et al, 2007). Under the fast, small and short-term [shorter than the regulation of enzyme concentrations through the gene expression loop] temperature variation the enzyme concentration in a biochemical system can be considered to be invariant because its adjustment takes longer time during which the temperature variation occurs. The aim of this research is to describe the temperature response of a steady state pathway flux to fast, small and short-term temperature change on the base of Metabolic Control Analysis (MCA)
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