Abstract
Serum concentrations of the antiepileptic drug gabapentin (GBP) are usually determined by high-performance liquid chromatography (HPLC) using UV photometric detection after pre-column derivatization with 2,4,6-trinitrobenzenesulphonic acid. Vigabatrin levels in serum are determined by HPLC using fluorescence detection. Like vigabatrin (VGB), gabapentin has also a primary amine group that easily reacts with o-phthaldialdehyde reagent and produces a fluorescing substance. By the use of fluorometric detection, GBP can be determined more simply, sensitively and simultaneously with VGB. The day-to-day coefficient of variation for the determination of GBP in a pooled serum was 4.0% ( n=17; serum concentration, 13.8 μmol/l) and for VGB was 3.1% ( n=21; serum concentration, 26.4 μmol/l). The lower limit of detection is 0.5 μmol/l for both drugs and the method is linear up to 500 μmol/l for GBP and 1300 μmol/l for VGB.
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