Abstract
An indirect competitive enzyme-linked immunosorbent assay (icELISA) and an immunochromatographic strip assay using a highly specific monoclonal antibody, were developed to detect methyltestosterone (MT) residues in animal feed. The optimized icELISA had a half-inhibition concentration value of 0.26 ng/mL and a limit of detection value of 0.045 ng/mL. There was no cross-reactivity with eight analogues, revealing high specificity for MT. Based on icELISA results, the recovery rate of MT in animal feed was 82.4%–100.6%. The results were in accordance with those obtained by gas chromatography-mass spectrometry. The developed immunochromatographic strip assay, as the first report for MT detection, had a visual cut-off value of 1 ng/mL in PBS, 2.5 ng/g in fish feed, and 2.5 ng/g in pig feed. Therefore, these immunoassays are useful and fast tools for MT residue detection in animal feed.
Highlights
Synthetic androgens have been used in sports and stock farming since the 1950s due to their effects on muscle strength and animal meat production [1]
MT in feed and its metabolite residues in meat can contribute to a series of adverse effects, several methods have been developed for the detection of MT residues
The most common detection methods are based on chromatography, including high performance liquid chromatography (HPLC) [10,11], liquid chromatography (LC) coupled with mass spectrometry (MS) [12], gas chromatography-mass spectrometry (GC-MS) [13,14] and LC-MS/MS [15,16]
Summary
Synthetic androgens have been used in sports and stock farming since the 1950s due to their effects on muscle strength and animal meat production [1]. The most common detection methods are based on chromatography, including high performance liquid chromatography (HPLC) [10,11], liquid chromatography (LC) coupled with mass spectrometry (MS) [12], gas chromatography-mass spectrometry (GC-MS) [13,14] and LC-MS/MS [15,16]. These methods are both specific and sensitive, but they require tedious sample preparation, complicated spectral analyses, and trained operators, making these methods unsuitable for field detection. ELISA, which has both high selectivity and adequate sensitivity, have been increasingly used in sample analysis [21,22,23]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
