Abstract
Tiamulin (TML) is a diterpenoid antibiotic used in animals. In this study, a gold nanoparticle immunochromatographic strip assay and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect the residue of TML in chicken. TML aminobutyric was synthesized and conjugated to keyhole limpet hemocyanin by mixed anhydride method as immunogen, whereas TML was connected to ovalbumin with 1,1′-carbonyldiimidazole as coating antigen. Under optimized conditions, the ultrasensitive monoclonal antibody-based ic-ELISA exhibited a half-maximal inhibitory concentration (IC50) value of 0.36 ng/mL with a working range of 0.14–0.9 ng/mL for TML. A rapid and sensitive immunochromatographic strip assay was developed with a TML cutoff value of 2.5 ng/mL. On the basis of these results, both developed methods are useful for TML detection in chicken.
Highlights
Tiamulin (TML), a diterpenoid compound, is one of the world’s top 10 used veterinary antibiotics with strong activity against mycoplasma, Streptococcus spp., and Staphylococcus aureus.[1−3] TML is widely used as a veterinary drug and feed additive to treat respiratory diseases and encourage growth in cattle.[4−6] TML, to other antibiotics, had been reported to have adverse effects on human health, which have been linked to drug residues found in food-animal tissues
There have been many methods developed for TML detection, including liquid chromatography−mass spectrometry,[7] high-performance liquid chromatography (HPLC),[8] ultraHPLC,[9,10] and gas chromatography
We described an ultrasensitive monoclonal antibody (mAb)-based assay against TML with a demonstrated IC50 value of 0.36 ng/mL
Summary
Tiamulin (TML), a diterpenoid compound, is one of the world’s top 10 used veterinary antibiotics with strong activity against mycoplasma, Streptococcus spp., and Staphylococcus aureus.[1−3] TML is widely used as a veterinary drug and feed additive to treat respiratory diseases and encourage growth in cattle.[4−6] TML, to other antibiotics, had been reported to have adverse effects on human health, which have been linked to drug residues found in food-animal tissues These residues may have the potential to contribute to the emergence of drug-resistant bacteria. There have been many methods developed for TML detection, including liquid chromatography−mass spectrometry,[7] high-performance liquid chromatography (HPLC),[8] ultraHPLC,[9,10] and gas chromatography These instrument-based analytical methods have low detection limits and good accuracy, they require skilled and trained labor and highly specialized equipment. A rapid and sensitive TML detection method is required
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