Abstract
An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame.
Highlights
Tomato (Solanum lycopersicum) is a food very rich in bioactive compounds such as vitamins or carotenoids and it contains a variety of phenolic compounds [1]
A wide range of methods have been reported for the detection of phenolic compounds in food, beverages, or biological samples [9,10,11,12], liquid chromatography coupled to mass spectrometry (LC-MS) [13,14,15,16,17,18] is the most commonly used technique due to its high sensitivity and selectivity
Declustering potential (DP), focusing potential (FP), entrance potential (EP), quantification and confirmation transitions with their corresponding collision energy (CE) were shown in Table 2 obtaining the optimum value for the mass conditions
Summary
Tomato (Solanum lycopersicum) is a food very rich in bioactive compounds such as vitamins or carotenoids and it contains a variety of phenolic compounds [1]. Phenolics play an important protective role in human health, decreasing mortality [2], cardiovascular disease [3], and DNA oxidation [4], and counteracting age-related cognitive decline [5]. An exhaustive identification of polyphenols in food and biological samples is of great interest due to their health-promoting effects. A wide range of methods have been reported for the detection of phenolic compounds in food, beverages, or biological samples (i.e., spectrophotometry, capillary electrophoresis, near-infrared spectroscopy, HPLC-UV-DAD) [9,10,11,12], liquid chromatography coupled to mass spectrometry (LC-MS) [13,14,15,16,17,18] is the most commonly used technique due to its high sensitivity and selectivity
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