Sensing of hyperprolinemia biomarker and its recognition in biological sample through “turn-on” event by Zn-based metal-organic framework

Abstract

A hydrogen bonded ability metal organic framework (MOF, 1Zn) is used for the ultra-sensitive “turn-on” detection of hyperprolinemia biomarker with ultrafast (within 5 s) colorimetric response making the first MOF based hyperprolinemia biomarker sensor. The detection limit (4.46 ppb) is outperformed compared to all contemporary hyperprolinemia biomarker based sensors. Further, the sensor showed the recognition of biomarker in biological sample (human saliva). The detection of biomarker is also realized through colorimetric response (solution based and paper strip method). The mechanism of sensing is established through the electron transfer and the absorption caused emission (ACE). Moreover, the theoretical study is performed to support the sensing mechanism. The control titration of 1Zn suggest that the free –NH2 group of linker in 1Zn is involved in supramolecular interaction (hydrogen bonding) with the carboxylic group present on biomarker results the facile occurrence of electron transfer and ACE. Consequently, the luminescence “turn-on” effect of 1Zn for hyperprolinemia biomarker is observed.