Abstract
After mobile phase optimization (composition and elution profile) on an analytical scale with a standard mixture of mono- and sesquiterpenoids, the prefractionation of an essential oil has been carried out by semipreparative liquid chromatography. Reversed-phase chromatography on an octadecyl-bonded silica was used and the separation of the essential oil into several fractions was achieved within 20 min in a single experiment. The fractions were oxygenated monoterpenes, monoterpene hydrocarbons, sesquiterpene hydrocarbons and oxygenated sesquiterpenoids. Thus, liquid chromatographic separations of essential oils can be achieved, at room temperature, avoiding the risk of thermal rearrangement and decomposition which can occur in gas chromatographic separations. The fractions obtained were easily analysed by gas chromatography—mass spectrometry and several minor components, in particular sesquiterpenoids, were identified.
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