Abstract
Rapid and simple monitoring of anticancer drug concentrations in blood is important for improving the efficacy of chemotherapeutic cancer treatment. In this study, we demonstrated the detection of 5-fluorouracil (5-FU) in human serum using a field-effect transistor (FET) biosensor with competitive adsorption between uncharged anticancer drugs and charged pseudo-targets. The target 5-FU and 5-FU-modified bovine serum albumin (BSA/5-FU) were competitively adsorbed on the FET sensor surface using antigen-antibody reaction with antigen-binding fragment (Fab) receptor. FET responses to the target 5-FU concentrations were obtained via changes in the amount of negatively charged BSA/5-FU captured by Fab molecules. In addition, the influence of serum components could be suppressed by diluting the samples with a nonionic surfactant solution. As a result, the FET biosensing system was capable of quantitatively detecting 5-FU concentrations in serum. Therefore, the semiconductor-based sensing system could enable the adjustment of anticancer drug dosages and potentially lead to improvements in anticancer drug therapy.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
