Abstract
Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerol from plant leaf tissue lipid extracts and the resolution and analysis of component molecular species. Sulfoquinovosyldiacylglycerol was resolved from phospholipids in a polar lipid fraction by isocratic normal phase HPLC with detection at 208 nm. The mobile phase was composed of heptane-isopropanol-0.001 M KCl 40:52:8 (v/v/v). Yields from spinach leaf lipid extracts were 1.8 mg.10 g-1 fresh wt leaf tissue. Molecular species components of purified sulfoquinovosyldiacylglycerol were separated by reversed-phase C18 HPLC, and fatty acid positional distribution was defined.
Highlights
Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerolfrom plant leaf tissue lipid extracts and the resolution and analysis of component molecular species
In previously developed analytical procedures for plant leaf lipids (15), SQDG was eluted from silicic acid in GL fractions together with MGDG and DGDG
Several laboratories have recently reported HPLC techniques for the direct isolation of lipid classes from total plant lipid extractions (16).This is obviously preferable over the need for a preliminary separation of nonpolar, GL, and PL lipid fractions
Summary
Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerolfrom plant leaf tissue lipid extracts and the resolution and analysis of component molecular species. Purification step may be required (2).Galactolipid fractions containing SQDG have been typically recovered from plant lipid extracts by silicic acid or acid-treated Florisil column chromatography and further resolved into lipid classes by TLC.
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