Self-pollen rejection in Brassica

Abstract

Evidence has accumulated for the Brassica self-incompatibility system, indicating that the cell wall-localized S locus glycoprotein (SLG) and the plasma membrane-anchored S receptor kinase (SRK) are necessary for the stigma to inhibit self-pollen. By introducing 55 kb of DNA-containing SLG, SRK and an anther-expressed gene into a self-compatible Brassica napus line, SLG and SRK have been shown to be sufficient for the female side but not for the male side of the self-incompatibility phenotype in Brassica. This suggests that there must be an independent pollen S factor encoded outside the cloned region. Proc. Natl. Acad. Sci. U. S. A. (2000) 97, 3713–3717.