Self-defense mechanisms of microorganisms from the antimicrobial effect of silver nanoparticles: Highlight the role of extracellular polymeric substances

Abstract

Silver nanoparticles (AgNPs) are nowadays widely utilized in various fields due to their unique antimicrobial properties. Extracellular polymeric substances (EPS) excreted by microorganisms might affect the transformations and antibacterial efficacy of AgNPs. In the present study, the effects of EPS released by Escherichia coli (E. coli) on the dissolution and sulfidation of AgNPs as well as the associated growth inhibition to E. coli were systematically investigated. The formation of EPS-corona caused the reduced exposure of (111) facets of AgNPs due to the preferential binding with aromatic protein components in EPS. The EPS inhibited AgNPs dissolution, while facilitated reductive transformation of the released Ag+ to Ag0 under simulated sunlight. Additionally, EPS enhanced the colloidal stability and reduced electrostatic repulsive of AgNPs, which favored the access of sulfide and significantly promoted the sulfidation of AgNPs under simulated sunlight, further reducing the available dissolved Ag+ ions. Consequently, the EPS relieved the antibacterial activity of AgNPs to E. coli. These findings highlight the importance of microbial EPS in the transformations and bactericidal effect of AgNPs, which provide clues for the development of AgNPs-based antibacterial strategies.