Abstract
Background: Lead contamination has turned into a major concern as it serves no useful purpose in the human body. Its presence in the body can lead to toxic effects on different organs. Aim: The purpose of the present study is to examine the efficacy of selenium in mitigating leadinduced oxidative stress and kidney injury in male albino Wistar rats. Further to study the underlying molecular mechanism. Methods: We hypothesized that selenium protect against lead nephrotoxicity by testing various parameters of kidney function in male rats treated for 28 days with o.5 mg/kg selenium versus control male rats that received a vehicle. Results: The results showed an increase of serum urea, creatinine, uric acid and urinary albumin in rats intoxicated with lead compared to control group. The increase of these parameters would indicate renal toxicity which is confirmed by histopathological changes. Lead intoxicated rats had significant increment in apoptosis as well as phosphorylated PERK, eIF2α and apoptotic proteins as CHOP, caspase 3 and 8 in the kidneys, which were attenuated by selenium treatment. Conclusion: This study clearly demonstrated that activation of PERK- eIF2α -CHOP signaling pathway was involved in lead induced nephrotoxicity in rats and selenium inhibited lead nephrotoxicity partly through suppression of PERK- eIF2α CHOP pathway.
Highlights
Lead (Pb), an environmental toxin, is commonly used in different fields, such as paints, ceramic and petroleum products
Effects of selenium treatment on the histological structures of the kidney in lead intoxicated rats: Histopathologically, kidney tissue appears normal architecture of the renal cell in both control and group treated with selenium alone
Effects of selenium treatment on MDA, total antioxidants, and caspase 3 and 8 in leadintoxicated rats: Lead intoxicated rats showed increase in renal MDA by 203% and decrease in renal Total Antioxidant Capacity (TAC) by 139% compared to normal control rats
Summary
Lead (Pb), an environmental toxin, is commonly used in different fields, such as paints, ceramic and petroleum products. Lead serves no useful purpose in the human body. Its presence in the body can lead to toxic effects on different organs, regardless of age, gender, or method of exposure. Lead contamination has turned into a major concern as it serves no useful purpose in the human body. Its presence in the body can lead to toxic effects on different organs. Results: The results showed an increase of serum urea, creatinine, uric acid and urinary albumin in rats intoxicated with lead compared to control group. The increase of these parameters would indicate renal toxicity which is confirmed by histopathological changes. Conclusion: This study clearly demonstrated that activation of PERK- eIF2α -CHOP signaling pathway was involved in lead induced nephrotoxicity in rats and selenium inhibited lead nephrotoxicity partly through suppression of PERK- eIF2α CHOP pathway
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