Abstract
Abstract— Total nucleic acids of rat brain have been separated by agarose gel chromatography at 2 m‐NaCl into DNA. transfer RNA plus low molecular weight RNA. and high molecular weight RNA fractions. The DNA fraction contained less than 1 per cent RNA by weight judged by either short‐term or long‐term labelling with ortho[32P]phosphate. The high molecular weight RNA fraction contained 28 s and 18 s ribosomal RNAs and a heterogeneous population of 20‐60 s RNAs, apparent after short‐term labelling and characterized by a high content of nearest‐neighbour‐labelled uridylic acid. The rapidly sedimenting (>30s) portion of these RNAs could be largely separated from ribosomal RNAs by gel filtration using 4% agarose. The ribosomal RNAs could be fully resolved into 28 s and 18 s components by agarose gel chromatography at 0.5 m‐0.6 m‐NaCl, as shown by analysis of their sedimentation and nucleotide composition.
Published Version
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