Selective Isolation of Component Myristicine from Ligustium sinense oliv by Second-Sphere Coordination Inclusion Crystallization

Abstract

The first ligand of [CoCl4 ]2 - and the second ligand of protonated N,N,N',N'-tetra-p-methyl- benzyl-ethylenediamine were used to construct a second-sphere coordination complex as the host framework. Myristicine was selectively isolated from the volatile oil of Ligustium sinense oliv by the inclusion crystalline method as the guest molecules. IR,NMR,X-ray diffraction were used to determine the structure of the inclusion complex,and the host-guest molar ratio in the complex was found to be 2∶ 1. The myristicine was isolated from the inclusion complex via Kugelrohr vacuum distillation technique and was characterized by IR, NMR and GC. According to the result of GC,the content of myristicine in the volatile oil of Ligustium sinense oliv is 60. 4% ,and the purity of isolated myristicine reaches 100% .