Selective Isolation and Screening of Actinobacteria Strains Producing Lignocellulolytic Enzymes Using Olive Pomace as Substrate.

Lamia Medouni-Haroune,Samia Azzouz,Sevastianos Roussos,Véronique Desseaux,Mouloud Kecha,Farid Zaidi,Sonia Medouni-Adrar

doi:10.15171/ijb.1278

Lamia Medouni-Haroune, Samia Azzouz + Show 5 more

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https://doi.org/10.15171/ijb.1278

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Abstract

Background Olive pomace, as the main by-product of the olive oil industry, is recently recycled as fermentation substrate for enzyme production. Objectives Actinobacteria isolates were separated from an Algerian soil under olive pomace cultivation and were evaluated for their lignocellulolytic enzymes production. Materials and Methods Isolates of Actinobacteria were separated from soils around oil mills using four isolation media, among them three were enriched by olive pomace. The isolates were screened for their cellulolytic, xylanolytic and ligninolytic activities. Isolates with potential of producing lignocellulose-degrading enzymes were selected under submerged fermentation based olive pomace. Results Ninety isolates of Actinobacteria were separated from soil samples. M3 medium (raw pomace autoclaved alone) was the best isolation medium (68 strains), whereas, the soil from oil mill with continuous system (S1) led to separation of 52 strains. Among the 90 isolates, 82 were shown promising enzyme activity, 19 isolates were presented the largest zone diameter (<30 mm). S1M3I and S1M3II isolates were exhibited the highest values. Conclusions Olive pomace with medium low cost and high titers of enzymes can be valorized by culture of Actinobacteria to produce lignocellulolytic enzymes for industrial applications.

Highlights

Olive pomace, as the main by-product of the olive oil industry, is recently recycled as fermentation substrate for enzyme production
Screening of carboxymethyl cellulases (CMCases), Xylanase and Laccase Producers A preliminary qualitative analysis was conducted by carboxymethyl cellulose (CMC) and xylan containing agar plates method described by Boroujeni et al [1] and guaiacol containing agar plates method described by Lu et al [8]
Actinobacteria isolates were separated from soil around olive oil mill

Summary

Introduction

As the main by-product of the olive oil industry, is recently recycled as fermentation substrate for enzyme production. Objectives: Actinobacteria isolates were separated from an Algerian soil under olive pomace cultivation and were evaluated for their lignocellulolytic enzymes production. Materials and Methods: Isolates of Actinobacteria were separated from soils around oil mills using four isolation media, among them three were enriched by olive pomace. Isolates with potential of producing lignocellulose-degrading enzymes were selected under submerged fermentation based olive pomace. M3 medium (raw pomace autoclaved alone) was the best isolation medium (68 strains), whereas, the soil from oil mill with continuous system (S1) led to separation of 52 strains. Conclusions: Olive pomace with medium low cost and high titers of enzymes can be valorized by culture of Actinobacteria to produce lignocellulolytic enzymes for industrial applications. Studies dealing with lignocellulolytic production by Actinobacteria using olive pomace residues are rare

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