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Abstract
We have previously determined the amounts of time required to complete various macromolecular synthetic processes needed for induction of allophanate hydrolase in Saccharomyces cerevisiae. This information provided a means of testing, in vivo, an early hypothesis suggesting that cycloheximide inhibited the initiation as well as elongation steps of protein synthesis. Our data suggest that initiation of protein synthesis in yeast may be inhibited by low concentrations of cycloheximide which do not significantly affect polypeptide chain elongation.
Highlights
We have recently determined the periods of time that are required to complete various macromolecular synthetic processes associated with induction of allophanate hydrolase [711]
The rnal gene product must be functional until 10 min; protein synthesis is initiated at 20 min and is terminated by 24 min
The results reported here suggest that such selective inhibition is possible
Summary
Portions of the culture quired to complete various macromolecular synthetic proc- were transferred to a nonpermissive condition for the function esses needed for induction of allophanate hydrolase in Sac- being tested. This was either an increased temperature, if the charomyces cerevisiae. We have recently determined the periods of time that are required to complete various macromolecular synthetic processes associated with induction of allophanate hydrolase [711] Appearance of this inducible, degradative enzyme in Saccharomyces occurs 26 min after addition of inducer (urea) at 15”. The results reported here suggest that such selective inhibition is possible
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