Selective excretion of yolk-derived tocotrienols into the bile of the chick embryo

Abstract

The aim of this study was to investigate the possibility of biodiscrimination between different forms of vitamin E during the development of the chick embryo. The vitamin E present in the initial yolk consisted of α-tocopherol (90%), ( β+ γ)-tocopherol (8%), α-tocotrienol (0.3%) and ( β+ γ)-tocotrienol (1.3%). In marked contrast, the vitamin E recovered from the bile of the day-16 embryo contained much higher proportions of α-tocotrienol (10%) and especially of ( β+ γ)-tocotrienol (42%). By the time of hatching, 56% of the vitamin E present in the bile was in the form of ( β+ γ)-tocotrienol. The residual yolk of the newly-hatched chick contained far greater proportions of α-tocotrienol (2.6%) and ( β+ γ)-tocotrienol (10%) than were present in the initial yolk. The results suggest that the liver of the embryo may selectively excrete tocotrienols as components of bile, whilst retaining the tocopherols within the hepatocytes. The increased proportions of tocotrienols in the residual yolk may result from the recycling of bile from the gall bladder to the yolk. The liver of the day-old chick contained α-tocopherol as the main form of vitamin E (90%) with only a small proportion (0.2%) of ( β+ γ)-tocotrienol. The α-tocopherol form was also the main vitamin E component in the brain (85%), heart (79%), lung (82%) and adipose tissue (91%) of the day-old chick. The present study suggests the occurrence of a high degree of biodiscrimination between tocopherols and tocotrienols during the development of the chick embryo.