Abstract
Herein, we report determination of dopamine (DA) at modified glassy carbon electrode (GCE) with a film produced by reduction of diazonium generated from p -nitroaniline (PNA). Pores were created purposely by stripping pre-deposited gold nanoparticles (AuNPs) in the modifier film. The modified electrodes were characterized electrochemically by common redox probes: hydroquinone (HQ), hexacyanoferrate [(Fe(CN)6)] 3- and hexamine ruthenium(III) [Ru(NH3)6] 3+ . Comparison was made for the cyclic voltammetric and amperometric response of DA using the modified electrodes against the bare GCE in phosphate buffer solution (PBS) of pH 7.5. The bare and modified GCE showed a linear response to DA in the concentration range of 0.2-2.2 mM and 5-35 µM with detection limit of 0.015 mM and 0.6112 µM, respectively. The modified electrode showed high sensitivity, well selectivity, good anti-interference ability, durable stability and good electrode reproducibility for determining DA. The reported modified electrode is a promising sensor for use in electroanalysis of DA. KEY WORDS : Diazonium, p -Nitroaniline, Gold nanoparticles, Dopamine, Ascorbic acid, Glassy carbon electrode Bull. Chem. Soc. Ethiop. 2017 , 31(3), 361-372 DOI: http://dx.doi.org/10.4314/bcse.v31i3.1
Highlights
Dopamine (DA) is a naturally occurring catecholamine that plays crucial role in the function of cardiovascular, central nervous, renal and hormonal systems and its anomalous concentration cause numerous problems like Parkinson’s disease, neurological disorder, drug addiction and Schizophrenia [1,2,3,4,5,6,7]
Electrochemical determination of DA has the challenge of eliminating interfering substances that exist together with DA in biological fluids
PNA film was grafted on bare glassy carbon electrode (GCE) from in situ generated diazonium and characterized electrochemically using the three redox probes
Summary
Dopamine (DA) is a naturally occurring catecholamine that plays crucial role in the function of cardiovascular, central nervous, renal and hormonal systems and its anomalous concentration cause numerous problems like Parkinson’s disease, neurological disorder, drug addiction and Schizophrenia [1,2,3,4,5,6,7]. PNA film was grafted on bare GCE from in situ generated diazonium and characterized electrochemically using the three redox probes. The redox peak of Fe(CN)63- significantly suppressed at PNA film-hole modified GCE (Figure 2A, b) relative to the bare GCE (Figure 2A, a).
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