Selective Electrochemical Capture and Release of Heparin Based on Amine-Functionalized Carbon/Titanium Dioxide Nanotube Arrays.

Abstract

Heparin (HEP) is a sulfated glycosaminoglycan that is a clinical anticoagulant agent. Commercially derived from porcine intestinal mucosa, HEP is challenging to separate from this complex biological mixture for additional purification. This study aimed to raise the purity of isolated HEP using electrochemical potential to increase its selective capture and release. We demonstrate an electrochemical platform featuring an anode composed of amine-functionalized carbon/titanium dioxide nanotube arrays on titanium foil (Ti/C-TNTAs-NH2) and a cathode made of expanded graphite. Our results show that Ti and Ti/C-TNTAs control plates do not adsorb HEP, even while applying an external potential to the cell. However, when the Ti/C-TNTAs electrode is modified by 3 aminopropyltriethoxysilane, the terminal NH2 groups provide a high density of positive charges that serve as binding sites, enabling the adsorption of HEP. This attraction is further strengthened by applying an external potential to the anode. Subsequent release of the HEP molecules and regeneration of the Ti/C-TNTAs-NH2 electrode are easily accomplished by applying an anodic potential to the plate, as well as by increasing the concentration of NaCl in solution. This electrochemical system demonstrates the good selectivity of HEP, even within a mixture of other probable interfering species (e.g., bovine serum albumin and chondroitin sulfate). Additionally, it maintains 90.11% of its initial electrosorption efficiency after ten repeated HEP adsorption/desorption cycles, indicating this system's promising stability and reusability for HEP purification.