Abstract
A glassy carbon electrode (GCE) modified with valine was used for the sensitive volt metric determination ofepinephrine (EP). The electrochemical response characteristics of the modified electrode toward EP, ascorbic acid (AA)and dopamine (DA) were investigated by cyclic voltammetry (CV). The results show an efficient catalytic activity ofthe electrode for the electro-oxidation of EP, which leads to an improvement to the reversibility of the electroderesponse. The effect of pH and potential sweep rate on the mechanism of the electrode process was investigated. Themodified electrode exhibits an efficient electron mediating behavior along with well-separated oxidation peaks for EP,AA, and DA. Under the optimum pH of 7.0 in phosphate buffer solution(PBS), the CV anodic peak current showed alinear relation versus EP concentration in the range of 4.5×10-6~1.0×10-5mol·L-1 and 1.0×10-5~1.4×10-4 mol·L-1, withcorrelation coefficients of 0.9997 and 0.9942. The detection limit is 7.6×10-7 mol·L-1. High sensitivity and selectivity,submicromolar detection limit, high reproducibility, along with ease of preparation and regeneration of the electrodesurface by simple polishing make this method suitable for the determination of EP in pharmaceutical and clinicalpreparations.
Highlights
Epinephrine (EP), often called adrenaline, is one of the most important neurotransmitters in mammalian central nervous systems and exists in the nervous tissue and body fluids
Determination of EP in the presence of ascorbic acid (AA) and DA, because of their coexistence in human fluids, is attractive to biological and analytical researchers. Individual determinations of these compounds have been reported in many references; simultaneous determination of them has always been considered as a serious challenge in these studies, an enormous amount of research has been devoted to the development of new modified electrodes for monitoring EP
The electrochemical detection of EP on the surface of bare electrodes has some fundamental problems, mainly high potential and sluggishness of the kinetics of the electrode process, which result in weak electrochemical responses Hernandez, 2001, PP.985–991).,in this paper, we described the preparation and suitability of valine modified glassy carbon electrode modified as a new electrocatalyst in the electrocatalysis and determination of EP in an aqueous buffer solution, we evaluated the analytical performance of the modified electrode in quantification of EP in the presence of AA and DA
Summary
Epinephrine (EP), often called adrenaline, is one of the most important neurotransmitters in mammalian central nervous systems and exists in the nervous tissue and body fluids. Determination of EP in the presence of AA and DA, because of their coexistence in human fluids, is attractive to biological and analytical researchers Individual determinations of these compounds have been reported in many references; simultaneous determination of them has always been considered as a serious challenge in these studies, an enormous amount of research has been devoted to the development of new modified electrodes for monitoring EP. The electrochemical detection of EP on the surface of bare (unmodified) electrodes has some fundamental problems, mainly high potential and sluggishness of the kinetics of the electrode process, which result in weak electrochemical responses Hernandez, 2001, PP.985991).,in this paper, we described the preparation and suitability of valine modified glassy carbon electrode modified as a new electrocatalyst in the electrocatalysis and determination of EP in an aqueous buffer solution, we evaluated the analytical performance of the modified electrode in quantification of EP in the presence of AA and DA. Valine was used as a new electrocatalyst; the scheme is shown in figure 1
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