Selective bioaccumulation of neonicotinoids and sub-lethal effects in the earthworm Eisenia andrei exposed to environmental concentrations in an artificial soil

Abstract

In this study, we evaluated the bioaccumulation of neonicotinoid insecticides in the earthworm Eisenia andrei exposed to environmental concentrations (<200 ng g−1 dry weight, nominal concentration) in an artificial soil. We tested the selectivity for neonicotinoids by exposing earthworms to 7 neonicotinoids alone and in more complex mixtures of 54 pesticides then 69 organic contaminants (OCs) (54 pesticides and 15 pharmaceuticals). We applied long-term (56-day) toxicity tests to further evaluate the effect of OCs on earthworms. We monitored adult survival, adult DNA damage using a comet assay on earthworm coelomocyte cells, and reproduction performance (i.e. number of cocoons and number and dry weight of juveniles). A selective bioaccumulation of neonicotinoid insecticides in adult and juvenile earthworms was found. This bioaccumulation is concomitant with a significant increase in adult DNA damage and significant effects on reproduction when earthworms were exposed to neonicotinoid insecticides alone. This study reveals a new potential point of entry of neonicotinoid insecticides into the wildlife food chain and also shows that E. andrei reproduction could be affected by long-term exposure to environmental concentrations of OCs.