Abstract
BackgroundRecent studies have revealed that destruxins (Dtx) have potent cytotoxic activities on individual cancer cells, however, data on oral cancer cells especial human are absent.MethodsDestruxin B (DB) was isolated and used to evaluate the selective cytotoxicity with human oral cancer cell lines, GNM (Neck metastasis of gingival carcinoma) and TSCCa (Tongue squamous cell carcinoma) cells, and normal gingival fibroblasts (GF) were also included as controls. Cells were tested with different concentrations of DB for 24, 48, and 72 h by MTT assay. Moreover, the mechanism of cytotoxicity was investigated using caspase-3 Immunofluorescence, annexin V/PI staining, and the expression of caspase-3, Bax, and Bcl-2 by western blotting after treated with different concentrations of DB for 72 h as parameters for apoptosis analyses.ResultsThe results show that DB exhibited significant (p < 0.01) and selective time- and dose-dependent inhibitory effects on GNM and TSCCa cells viability but not on GF cells. The data suggested that DB is capable to induce tumor specific growth inhibition in oral GNM and TSCCa cancer cells via Bax/Bcl-2-mediated intrinsic mitochondrial apoptotic pathway in time- and dose-dependent manners.ConclusionsThis is the first report on the anti-proliferation effect of DB in oral cancer cells. The results reported here may offer further evidences to the development of DB as a potential complementary chemotherapeutic target for oral cancer complications.
Highlights
Recent studies have revealed that destruxins (Dtx) have potent cytotoxic activities on individual cancer cells, data on oral cancer cells especial human are absent
The selective cytotoxicity of Destruxin B (DB) was examined in human oral squamous cell carcinoma cell lines GNM (Neck metastasis of gingival carcinoma) and TSCCa (Tongue squamous cell carcinoma) cells, as well as the normal gingival fibroblast (GF) cells
The results showed that DB exhibited significant (P < 0.05) time- and dose-dependent inhibitory effects on GNM and TSCCa cells viability
Summary
Recent studies have revealed that destruxins (Dtx) have potent cytotoxic activities on individual cancer cells, data on oral cancer cells especial human are absent. Destruxins, especially Destruxin A, B and E (DA, DB, and DE), are a class of insecticidal cyclic depsipeptides [2]. Previous studies have shown destruxins exhibited strong biological effects; for example, destruxins disturbs macromolecular syntheses (DNA, RNA and protein synthesis) [3], produces anti-hepatitis B effects [4,5,6] and modifies the DNA content of murine leukemia cells [7,8,9] in vitro. In the battle against cancer, one of the main technologies used for treatment is chemotherapy.
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