Selective and fast removal and determination of β-lactam antibiotics in aqueous solution using multiple templates imprinted polymers based on magnetic hybrid carbon material

Abstract

Selective and fast adsorption of five broad-spectrum β-lactam antibiotics included amoxicillin, cephalexin, cefazolin, penicillin G and oxacillin was achieved by novel surface molecular imprinting polymers (MIPs) with magnetic hybrid carbon material as the substrate. The characteristics of MIPs were studied by scanning electron microscope, Fourier transform infrared spectrometer, thermogravimetric analysis, etc. And through a series of adsorption experiments to examine the kinetics, isotherms, thermodynamics, selectivity and reusability for sorption of β-lactam antibiotics onto the MIPs. The adsorption equilibriums were accomplished in about 60 min with adsorption capacities of 4.57–24.55 mg g−1, while the adsorption process was preferably fitted with pseudo-second-order kinetic model and Freundlich model. The imprinting factors ranged from 1.88 to 9.94 indicated the MIPs possessed excellent recognition ability, and its good reusability was demonstrated after five times adsorption–desorption cycles without a large drop in adsorption capacity. Furthermore, a new detection method was developed by combining of MIPs with solid phase extraction and high performance liquid chromatography (MIPs-SPE-HPLC). Under the optimal SPE conditions, the limits of detection (LODs) for the five BLAs were 0.24–0.56 µg L−1, with RSDs of 0.76–5.39%. The synthesized MIPs and the proposed MIPs-SPE-HPLC method can be applied for the efficient, simultaneous separation and detection of BLAs.