Abstract
Soilless culture of valuable cultivars of grapevine as 'Feteasca Alba', 'Italian Riesling', 'Pinot Gris' and derived clones, was accomplished by culture of shoot tip explants on MS media supplemented with growth regulators. In vitro culture was induced after estimation of genetic polymorphism in their populations by RAPD markers. The amplification was accomplished by using 26 primers and the specific working programme for RAPD. Genetic polymorphism in the populations was oberved by RAPD markers obtained by DNA amplification with OPB-04, OPB-07, OPB-12, OP-17, OPF-04, OPA-13 primers. Plants showing the most polymorphic RAPD pattern were cultivated in vitro. Regenerated plants from shoot tip explants were obtained on MS media supplementd with 5.0 μM 2iP, but multiplication is low. Rooting of in vitro plantlets was obtained on MS media supplemented with 0.5 μM IBA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
