Selection of potential Indonesian plant species for antioxidant

K Kartini,F Setiawan,O Yunita,J Sukweenadhi,C Avanti

doi:10.1088/1755-1315/457/1/012040

K Kartini, F Setiawan + Show 3 more

Open Access

https://doi.org/10.1088/1755-1315/457/1/012040

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Abstract

Diabetic foot ulcer (DFU) is commonly complication of diabetes mellitus. Antimicrobial and antioxidant herbs can be used as complementary therapy in DFU and these properties will be strengthened by silver nanoparticles (AgNPs). This study was performed for screening of Indonesian herbs promising as antioxidant which can be subsequently involved in the green synthesis of AgNPs. Antioxidant of eight herbs extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl method. Their total group of compounds were quantified as well. Phyllanthus niruri and Orthosiphon stamineus exhibited the strongest antioxidant power with IC50 of 102 and 133 μg/ml, followed by Curcuma domestica, Stelechocarpus burahol, and Curcuma xanthorriza with the IC50 of 363, 481, and 540 μg/ml, respectively. Sonchus arvensis, Apium graveolens, Centella asiatica did not have antioxidants activity. Total phenolics from the highest is: O. stamineus > C. domestica > C. xanthorrizha > P. niruri > S. burahol > S. arvensis > A. graveolens > C. asiatica, whereas total flavonoids order is O. stamineus > S. burahol > P. niruri > A. graveolens > C. asiatica > S. arvensis > C. xanthorrizha > C. domestica. We concluded that O. stamineus was the most suitable herb to be used as a bioreductor in the green synthesis of AgNPs.

Highlights

A high prevalence of diabetes causes the emergence of comorbidities
Total phenolics was analysed with the Folin-Ciocalteu method, whereas total flavonoids was evaluated with the aluminum chloride (AlCl3) colorimetric method using spectrophotometer
Phyllanthus niruri and Orthosiphon stamineus showed the most potent antioxidant with an IC50 value of 102 and 133 μg/ml, followed by C. domestica, S. burahol, and C. xanthorriza with the IC50 value of 363, 481, and μg/ml ppm, respectively

Summary

Introduction

A high prevalence of diabetes causes the emergence of comorbidities. Diabetes is the cause of non-traumatic lower limb amputation, which is preceded by wounds that do not heal [2]. The risk of wound in diabetics is 15-20% [3]. About 15-27% of diabetic patients experience amputation due to infection [4]. 45-60% of diabetic foot ulcers (DFU) are estimated due to neuropathy [5]. Emerging studies show high infections of Pseudomonas [6], E. coli [7], and S. aureus [8] in DFU. Diabetic foot ulcers treatment requires handling of wounds as well as giving appropriate antibiotics [1]. Silver/sulphadiazine dressing can be considered in DFU therapy. Silver and polyherbal dressing shows promising results in wound healing [9]

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