Process Optimization for Green Synthesis of Silver Nanoparticles Using Indonesian Medicinal Plant Extracts

Kartini Kartini,Johan Sukweenadhi,Dia Anjarwati,Christina Avanti,Amarisa Alviani,Adinda Finna Fanany

doi:10.3390/pr8080998

Kartini Kartini, Johan Sukweenadhi + Show 4 more

Open Access

https://doi.org/10.3390/pr8080998

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Journal: Processes	Publication Date: Aug 17, 2020
Citations: 16	License type: CC BY 4.0

Affiliation: University of Surabaya

Abstract

Silver nanoparticles (AgNPs) are an interesting metal nanoparticle that can be incorporated into pharmaceutical products, including for diabetic foot ulcers as an antimicrobial agent. Green synthesis of AgNPs using plant extracts has been drawing much attention as it is simple, eco-friendly, stable, and cost-effective. This present study was performed to evaluate the potential of three Indonesian medicinal plant extracts, namely Phyllanthus niruri (PN), Orthosiphon stamineus (OS), and Curcuma longa (CL), as reducing and capping agents in the green synthesis of AgNPs, and to optimize their concentrations. Based on the yields and characteristics of the formed nanoparticles, which were analyzed using a UV-Vis spectrophotometer, particle size analyzer, scanning electron microscope, and X-ray diffractometer, Phyllanthus niruri extract at a concentration of 0.5% was concluded as the best extract in the green synthesis of AgNPs. It is thereby a prospective reducing and capping agent for further scale-up studies.

Highlights

Diabetic foot ulcers (DFUs) are one of the common complications of diabetes mellitus.The wound healing process in this disorder is much more delicate than normal wounds and can be inhibited by the presence of oxygen-free radicals, microbial infection, and high blood glucose.The microbial burden is believed to underlie the delayed healing process in DFU [1], and Staphylococci, Pseudomonas, Citrobacter, and Enterococci are major bacteria colonizing diabetic wounds [2]
Crude drugs consisting of the aerial parts of Phyllanthus niruri, Orthosiphon stamineus leaves, and Curcuma longa rhizomes, provided by a cultivation and research center for medicinal plants in Tawangmangu City, Indonesia, were collected in June 2019
An environmental-friendly and low-cost synthesis method of AgNPs using three Indonesian plant extracts commonly used as traditional medicines has been conducted

Summary

Introduction

Diabetic foot ulcers (DFUs) are one of the common complications of diabetes mellitus.The wound healing process in this disorder is much more delicate than normal wounds and can be inhibited by the presence of oxygen-free radicals, microbial infection, and high blood glucose.The microbial burden is believed to underlie the delayed healing process in DFU [1], and Staphylococci, Pseudomonas, Citrobacter, and Enterococci are major bacteria colonizing diabetic wounds [2]. Diabetic foot ulcers (DFUs) are one of the common complications of diabetes mellitus. The wound healing process in this disorder is much more delicate than normal wounds and can be inhibited by the presence of oxygen-free radicals, microbial infection, and high blood glucose. The microbial burden is believed to underlie the delayed healing process in DFU [1], and Staphylococci, Pseudomonas, Citrobacter, and Enterococci are major bacteria colonizing diabetic wounds [2]. Ulcer treatment requires comprehensive management, including the use of appropriate antibiotics. Natural polymers dressing, have exhibited promising results in diabetic wound healing [3,4]. Silver ions have been recognized for decades as active antimicrobial and tissue regenerating agents

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Conclusion