Abstract
BackgroundOleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-called second generation biofuels, which are based on non-food competing “waste carbon sources”.ResultsIn this study the selection of potentially new interesting oleaginous yeast strains is described. Important selection criteria were: a broad maximum temperature and pH range for growth (robustness of the strain), a broad spectrum of carbon sources that can be metabolized (preferably including C-5 sugars), a high total fatty acid content in combination with a low glycogen content and genetic accessibility.ConclusionsBased on these selection criteria, among 24 screened species, Schwanniomyces occidentalis (Debaromyces occidentalis) CBS2864 was selected as a promising strain for the production of high amounts of lipids.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-016-0276-7) contains supplementary material, which is available to authorized users.
Highlights
Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs)
Fatty Acid Methyl Esters originating from the lipids of oleaginous micro-organisms show identical fuelling properties compared to conventional diesel and could be used in modern cars without major adaptations [8]
It was shown that lipid accumulation in R. toruloides is observed at a C/N ratio of 30 and increases with an C/N ratio up to 120 using glucose as carbon source [21]
Summary
Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). Fatty Acid Methyl Esters originating from the lipids of oleaginous micro-organisms (e.g. algae, yeast and fungi) show identical fuelling properties compared to conventional diesel and could be used in modern cars without major adaptations [8]. Oleaginous yeasts should preferably be able to grow to high cell densities combined with a high fatty acid content, have good growth characteristics at low pH and a broad temperature range
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