Abstract
Selection of a sublibrary enriched for a chromosome from total human bacterial artificial chromosome library using DNA from flow sorted chromosomes as hybridization probes
Highlights
The division of total genomic libraies into chromosome-specific A sublibraries is critical for efficient analysis and mapping of the genome
bacterial artificial chromosome (BAC) library gridded onto 42 hybridization filters at 5 x 5 density using a Biomekl,000 'gndding tool [2]. 100 ng of sorted DNA
The total library was gridded on 42 separate filters
Summary
The division of total genomic libraies into chromosome-specific A sublibraries is critical for efficient analysis and mapping of the genome. BAC library gridded onto 42 hybridization filters at 5 x 5 density using a Biomekl,000 'gndding tool [2]. We hybridized the library filters with sorted chromosome 21 s Comparison of hybridization results from both chromosomal DNA allowed us to identify many of the Fge 1.
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