Abstract
Summary The concentration of inorganic ions (Na + , K + +, Ca + + , Mg + + , Cl - , and NO 3 - ) in seedlings, morphogenetic calli and somaclones from three Canary Island (Spain) tomato land races was analyzed. MS medium supplemented with 1.2, 2.4, 3.6, and 5.8 g/1 NaCl were used. Raising the NaCl concentration of the media increased the endogenous concentration of Na + and Cl - in all plant material analyzed. Na + and Cl - concentration were higher in morphogenetic calli than in seedlings and higher in somaclones than in the morphogenetic calli from which they were derived. The decrease in NO 3 - ) and K + did not correlate with the increase in Na + and CI - . Calcium and Mg + + contents did not vary significantly with NaCl increase. The tolerance of the selected phenotypes seems to be related to a mechanism that controls the internal osmotic potential by increasing CI - and Na + concentrations while preventing the cells from their toxic effect.
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