Abstract
The sensitivity of catecholamines using selected-ion monitoring gas chromatography—mass spectrometry (SIM GC—MS) has been enhanced by employing a simple clean-up step using Sephadex G-10 columns. This procedure allows for the detection of extremely small amounts (1–10 pg) of biogenic amines (e.g., epinine) which corresponds to a 25-fold increase in detection limits compared to electron ionization GC—MS or high-performance liquid chromatography with electrochemical detection (HPLC—ED). The SIM GC—MS assay was used to monitor the dopamine-β-hydroxylase-mediated conversion of epinine d6 to epinephrine d6 in rat hypothalamus and brainstem in vitro and the results were compared to those obtained by HPLC—ED.
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