Seed priming of stored seeds on seed germination, vegetative performance, flowering, and proline content in chili under water stress condition

Abstract

Chili seeds mostly have been stored for a long period; therefore, the seed quality may be affected. Prior to planting, reinvigoration by priming treatments becomes an important treatment. The purpose of this study was to evaluate how priming affected seed germination, vegetative performance, flowering, and proline content in chili cv. "Inata Agrihorti" under water stress treatment. The research was carried out in the Physiology Laboratory and Shade House in Batu East Java, Indonesia from December 2022 to March 2023, A factorial Randomized Block Design with three replications was set up in this study that consisted of two factors. First factor was stored seeds (2 and 5 years) duration of chili cv. Inata Agrihorti, and the second factor was priming treatments (Phenol Ethyl Glycol 6000, Gibberellic acids, Phenol Ethyl Glycol 6000 + Gibberellic acids, and distilled water as a control). The reinvigoration of stored seeds by priming treatments significantly improved germination percentage, germination time, root length, and proline content of early seedlings’ stages either under water stress or distilled water treatment as a control. The interaction of priming treatment and stored seeds was also recorded in improving the vegetative performance, flower number, and proline content of chili plants derived from reinvigorated seeds under water stress or normal watering treatment. Under water stress and/or normal conditions, primed seeds of 2-year-old stored seeds produced larger values of these variables than primed seeds of 5-year-old stored seeds. To improve yield, seed invigoration of stored seeds of chili cv Inata Agrihort through - 1.5 MPa Phenol Ethyl Glycol 6000 treatment was recommended.