Abstract
Seed oils from Pterocarya stenocarpa (Juglandaceae), Morus bombycis (Moraceae), Ficus pumila (Moraceae), Humulus japonicus (Moraceae) and Distylium racemosum (Hamamelidaceae), were analyzed for their characteristics (Table-1). The bromination test on the fatty acids indicated the presence of linoleic acid in each oil and linolenic acid in each oil except M. bombycis oil.The fatty acid methyl ester prepared from each oil by the routine method was analyzed by GLC.The triglyceride fraction of each oil was separated by column chromatography using silica gel, and then partially hydrolyzed with a pancreatic lipase. The 2-monoglyceride fraction of the hydrolysed product was separated by TLC, and converted into the methyl esters. The methyl esters were then analyzed by GLC. Thus, all fatty acid compositions in the sample oils and the 2-position of triglyceried were contrasted (Table-2).
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