Seed germination in Narcissus yepesii (Amaryllidaceae): clinal variation in the morphophysiological dormancy levels.

Abstract

Seed dormancy classes determine both population and species-level processes which can be crucial in the life cycle of many plants. However, there are no studies of a dormancy cline between levels of morphophysiological dormancy (MPD). We aimed to determine the class of seed dormancy of Narcissus yepesii exhibits in order to explore links between different dormancy levels, previously characterized in two closely related phylogenetic congeners, N. alcaracensis and N. longispathus. Experiments were carried out under both near-natural temperature and controlled laboratory conditions. The parameters calculated were mean embryo length, radicle and shoot emergence percentages. The effects of different periods of storage; and different periods with or without GA3 of warm, cold or warm plus cold were analysed. The Narcissus populations from the Baetic System of mountain ranges in south-eastern Spain show clinal variation in a northeast–southwest gradient from intermediate to non-deep complex MPD, through the coexistence of intermediate and non-deep complex MPD in N. yepesii (21 % and 74 %, respectively). In addition, 54 % of stored seeds were able to show both levels of MPD. Narcissus yepesii occupies an intermediate position between N. alcaracensis and N. longispathus in the geographical distribution and in the clinal germination ranges. It strongly suggests an evolutionary gradient, which connects the intermediate complex MPD with the non-deep complex MPD in southern Iberian daffodils. This is the first study showing a gradient in the evolution between levels of MPD. Our results demonstrate a cline in these levels in response to both an environmental gradient and genetic differences.