Abstract
This research endeavors to inspect the chemical and biological profiling of methanol and dichloromethane (DCM) extracts prepared from Abutilon figarianum Webb. Total bioactive constituents and secondary metabolites were assessed via ultra-high performance liquid chromatography (UHPLC-MS). Biological effects were evaluated via antioxidant and enzymes inhibitory assays. The methanol extract was able to give the highest phenolic (51.92 mg GAE/g extract) and flavonoid (72.59 mg QE/g extract) contents and was found to contain 11 bioactive metabolites, including flavonoid, alkaloid, phenolic and fatty acid derivatives, as accessed by UHPLC-MS analysis. Similarly, the phytochemical profiling of the DCM extract tentatively identified the 12 different secondary metabolites, most of these were fatty acid derivatives. The methanol extract was most active in the radical scavenging, reducing power and total antioxidant power assays, while dichloromethane extract showed the highest metal chelating activity. For enzyme inhibition, the DCM extract showed the highest activity against cholinesterases, glucosidase and amylase, whereas methanol extract was most active against tyrosinase. Docking studies have supported the observed biological activity, where isobergapten showed higher activity against tyrosinase (−7.63 kcal/mol) with inhibition constant (2.55 µM), as opposed to other enzymes. The observed antioxidant and inhibitory potentials of A. figarianum against the studied enzymes tend to endorse this plant as a prospective source of bioactive phytochemicals.
Highlights
IntroductionPlants have been used as a potential source of medicines
For hundreds of years, plants have been used as a potential source of medicines
It was observed that the plant contains a higher amount of flavonoids and these results show similarity with the earlier study conducted by Srividya et al (2012), who found the highest flavonoid content as compared to phenolic in different extracts of A. indicum [23]
Summary
Plants have been used as a potential source of medicines. People are dependent on plants for a panoply of uses such as food, wood, therapies, timber and non-timber forest products [1]. Plants are always considered as a potential source of biologically active drugs and have various traditional uses for the service of humanity since time immemorial [2]. A considerable amount of antioxidants, like polyphenols, is present in medicinal plants, which have a significant role in adsorption and neutralization of harmful free radicals. Antioxidants from herbal sources play a vital role in protection from various health disorders such as skin problems, diabetes mellitus, different types of cancers, Parkinson’s (PD), Alzheimer’s disease (AD) and oxidative stress [3]. Incidents of Alzheimer’s disease, which can be linked to aging, is almost going to reach 81.1 million in 2040 [5]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.