Seasonal Variations in the Antioxidant and Phytochemical Composition of Five Wild Edible Plants from Paschim Medinipur: Implications for Nutritional Value

Abstract

Seasonal fluctuations in antioxidant and phytochemical composition of five wild edible plants (WEPs): Amla (Emblica officinalis), Jamun (Syzygium cumini), Water Spinach (Ipomoea aquatica), Karonda (Carissa spinarum) and Ashwagandha (Withania somnifera) were investigated in Paschim Medinipur, India. Samples were collected across four seasons: monsoon, post-monsoon, winter, and pre-monsoon. TPC, TFC, and DPPH assays were performed. High-performance liquid chromatography (HPLC) profiled key phytochemicals. This study delves into this captivating tango, seeking to: Unravel the seasonal variations in the antioxidant and phytochemical composition of these five WEPs. This exploration encompasses quantifying total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (DPPH) across four distinct seasons: monsoon, post-monsoon, winter, and pre-monsoon; Decipher the identity and abundance of key phytochemicals within them. By employing high-performance liquid chromatography (HPLC), we aim to unveil the unique chemical tapestry woven in each WEP, revealing the spectrum of phenolics, flavonoids, and carotenoids that contribute to their nutritional potency; Evaluate the impact of seasonal variations on the overall nutritional value of WEPs. By linking the fluctuations in antioxidants and phytochemicals to the changing seasons, we hope to understand how to optimize the consumption of these precious bioactives, empowering communities to embrace the full nutritional potential of their local flora. TPC, TFC, and DPPH activity varied significantly across seasons (p<0.05) in all WEPs. Highest antioxidant activity was observed in Jamun (Syzygium cumini) during post-monsoon (DPPH IC50=6.2 μg/mL) and lowest in Amla (Emblica officinalis) during pre-monsoon (DPPH IC50=22.5 μg/mL). HPLC identified phenolics, flavonoids, and carotenoids, with highest diversity in Ashwagandha (Withania somnifera). WEPs exhibited higher antioxidant potential during wetter seasons, coinciding with peak phytochemical levels, suggesting a defense mechanism. This study highlights the seasonality of antioxidant and phytochemical composition in WEPs, impacting their nutritional value. Consumption across seasons could optimize daily intake of these bioactives, contributing to a healthy diet in Paschim Medinipur. Further research on in vitro and in vivo bioactivity is recommended.