Abstract
The four major flavonoid aglycones (apigenin, luteolin 7-methyl ether, kaempferol 3-methyl ether, quercetin 3,3'dimethyl ether) in the external leaf resin of Isocoma acradenia were examined monthly over a one year period. Ten plants in one population were sampled repeatedly; each month 10 leaves per plant were collected (i.e. one leaf each from the tenth node of ten stems). Flavonoids were examined using reverse phase HPLC in acetonitrile and water. No qualitative variation was found among the four flavonoids. Analysis of variance of integrated peak areas revealed significant seasonal variation in relative per cent composition among apigenin, luteolin 7-methyl ether and quercetin 3,3'-dimethyl ether. Kaempferol 3-methyl ether did not vary significantly. The flavonoid aglycone composition showed no clear relationship with the amount of exudate.
Published Version
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