Seasonal Variation in Gut Microbiota Related to Diet in Fejervarya limnocharis.

Wenbo Liao,Chunhua Huang

doi:10.3390/ani11051393

Abstract

Simple SummaryGut microbiota, such as Proteobacteria, Firmicutes, and Bacteroidetes, show chitin-degrading and/or antibiotic biosynthesis ability. To evaluate whether ecological factors (diet) and host conditions (body size, body mass, and body condition) affect the gut microbiota diversity, we analysed the diet composition and host condition in Fejervarya limnocharis among different seasons and/or sexes. The dietary difference was not significant among seasons and between males and females, but host condition seasonally changed. Using bioinformatics analysis, we observed that food variations and body mass were significantly correlated with gut microbial composition. Our findings suggest that gut microbiomes of F. limnocharis vary seasonally in response to diet variations when facing environmental changes.Organisms adapt to environmental fluctuations by varying their morphology and structural, physiological, and biochemical characteristics. Gut microbiome, varying rapidly in response to environmental shifts, has been proposed as a strategy for adapting to the fluctuating environment (e.g., new dietary niches). Here, we explored the adaptive mechanism of frog intestinal microbes in response to environmental changes. We collected 170 Fejervarya limnocharis during different seasons (spring, summer, autumn, and pre-hibernation) to study the compositional and functional divergence of gut microbiota and analysed the effects of seasonal feeding habits and body condition on intestinal microorganisms using 16S rRNA high-throughput sequencing, Tax4Fun function prediction analysis, and bioinformatics analysis. The results showed no significant dietary difference in various seasons and between males and females. However, a significantly positive correlation was detected between dietary diversity and food niche width. Host condition (body size, body mass, and body condition) also revealed seasonal changes. The frogs were colonised by 71 bacterial phyla and dominated by Proteobacteria, Firmicutes, and Bacteroidetes. Stenotrophomonas was the most abundant genus in the Proteobacteria. The composition, diversity, and function of intestinal microorganisms in different seasons were significantly different. Significant differences were observed in composition and function but not in the microbial diversity between sexes. Furthermore, seasonal foods and body mass were significantly correlated with gut microbial composition. Our results suggest that gut microbiomes of F. limnocharis vary seasonally in response to diet under fluctuating environments.

Highlights

Animals evolve a variety of behavioral and physiological strategies, including altered feeding and activity patterns and increased mobilization of stored fat to fuel energy demands, to cope with shifting demands [1]
Chitinolytic bacteria (e.g., Clostridium) from tadpoles are advantageous in the digestion of insects, which are the main diet of anurans [4]
The V4 region of bacterial 16S rRNA gene in intestinal microbiota collected from 161 frogs across four seasons was sequenced to characterize microbiotas of F. limnocharis

Summary

Introduction

Animals evolve a variety of behavioral and physiological strategies, including altered feeding and activity patterns and increased mobilization of stored fat to fuel energy demands, to cope with shifting demands [1]. The gut microbiome has been proposed as an additional avenue by which animals can cope with changing dietary landscapes and energetic challenges [2,3] and play a role that has profound ecological, evolutionary, and environmental implications in amphibians for the most part [4]. Diverse and abundant microbial communities colonise vertebrate digestive systems and coevolve with the host [5,6], which affects multiple aspects of host physiology, including health, nutrition, immunity, development, and reproduction [7,8]. Important energetic gains made by tadpoles from a fermentative microbial digestion within their guts and these gains, in turn, are expected to be directly proportional to metamorphic size and/or timing [4]. Antibiotic genes of intestinal microorganisms encode the biosynthesis of antibiotics to affect the intestinal microflora and its antimicrobial and anti-inflammatory effects [9]

Methods

Results

Discussion

Conclusion