Abstract
Here we provide seasonal data on the concentrations of total coliform, Escherichia coli and Enterococcus spp. and six opportunistic pathogens (Acanthamoeba spp., Legionella spp., Legionella pneumophila, Mycobacterium avium, Mycobacterium intracellulare, and Pseudomonas aeruginosa) of public health significance in 24 tank water samples over six monthly sampling events from August 2015 to March 2006. Quantitative PCR (qPCR) assays were chosen for the quantification of six opportunistic pathogens and culture-based methods were used for the enumeration of fecal indicators. The data fle has been stored in a publicly available repository. The data on concentrations of opportunistic pathogens in RHRW will provide information for rainwater users regarding potential seasonality of risks. Quantitative data presented in this study can be used to perform quantitative microbial risk assessment (QMRA) of RHRW for various potable and nonpotable uses. Data can be used by health regulators to develop guidelines related to RHRW. Funding statement: This research was undertaken and funded as part of a Fulbright-CSIRO Postgraduate Scholarship sponsored by the CSIRO Land and Water Flagship.
Highlights
Mycobacterium avium Pseudomonas aeruginosa Mycobacterium intercellulare minimize Quantitative PCR (qPCR) contamination, DNA extraction and qPCR setup were performed in separate laboratories
Quantitative qPCR assays were used to measure the concentrations of six opportunistic pathogens in 24 Roof-harvested rainwater (RHRW) tanks over six months
Sampling strategy Monthly water samples were collected from 24 roof-harvested rainwater tanks on six separate events from August 2015 to March 2016, giving a total number of 144 tank water samples
Summary
We provide seasonal data on the concentrations of total coliform, Escherichia coli and Enterococcus spp. and six opportunistic pathogens (Acanthamoeba spp., Legionella spp., Legionella pneumophila, Mycobacterium avium, Mycobacterium intracellulare, and Pseudomonas aeruginosa) of public health significance in 24 tank water samples over six monthly sampling events from August 2015 to March 2006. The data on concentrations of opportunistic pathogens in RHRW will provide information for rainwater users regarding potential seasonality of risks. We provide seasonal data on the concentrations of fecal indicators (total coliform, Escherichia coli and Enterococcus spp.) and six opportunistic pathogens (Acanthamoeba spp., Legionella spp., Legionella pneumophila, Mycobacterium avium, Mycobacterium intracellulare, and Pseudomonas aeruginosa) of public health significance in tank water over six monthly sampling events. Ahmed et al: Seasonal Abundance of Fecal Indicators and Opportunistic Pathogens in Roof-Harvested Rainwater Tanks. The minimum information for publication of quantitative real-time PCR experiments (MIQE) guidelines were used throughout the qPCR quantification of opportunistic pathogens [8]
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