Abstract
This chapter reviews a study conducted for searching pro-drug substances from natural resources by supercritical carbondioxide extraction and bioassay tests. In this study, supercritical fluid extraction (SFE) was combined with five types of bioassay tests to explore some bioactive substances from thirty types of natural resources available in Korean peninsula. Employed bioassays include cytotoxicity, bleb forming, DNA binding, oxygen-free radical scavenger, and xanthine oxidase inhibitor tests. To evaluate comparatively the economic viability of the SFE, organic liquid–solvent extraction (LSE) with n-hexane, chloroform, and methanol was also performed. To characterize the extracts, gas chromatography (GC) and high performance liquid chromatography (HPLC) are employed. To obtain the high-pure compounds from the candidate total extracts, column chromatographic separation is performed for some illustrative total extracts. For all the samples, the optimum SFE condition for each sample that gives maximum yield and cytotoxicity are discussed in the chapter. For many sample resources, the SFE is found to be advantageous over the LSE.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
